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J Vet Intern Med. 2008 Sep-Oct;22(5):1090-4. doi: 10.1111/j.1939-1676.2008.0147.x. Epub 2008 Jul 11.

Detection of Mycobacterium avium subspecies paratuberculosis-specific DNA by PCR in intestinal biopsies of dogs.

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1
Clinic for Small Animals, Internal Medicine, Justus-Liebig-University of Giessen, Giessen, Germany.

Abstract

BACKGROUND:

Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of paratuberculosis. MAP infections have not been reliably detected in dogs, but a reemerging debate about the link between MAP and Crohn's disease has renewed interest about the occurrence of MAP in pets.

HYPOTHESIS:

This study was undertaken to examine canine intestinal biopsies for the presence of MAP-specific DNA.

ANIMALS:

Forty-two dogs with chronic vomiting, diarrhea, or both; and 14 dogs with no gastrointestinal disease.

METHODS:

All dogs with signs of gastrointestinal disease had a standard work-up for chronic gastrointestinal disease. Endoscopically obtained intestinal biopsies were submitted for histopathologic and molecular investigations. Biopsies were screened for MAP-specific DNA by 3 polymerase chain reaction (PCR) methods (nested, seminested, and triplex real-time PCR). Samples from control dogs were obtained during necropsy.

RESULTS:

Histopathology of the biopsies was indicative of inflammatory bowel disease (IBD) in 17 and neoplasia in 6 dogs. Six dogs showing nonspecific changes responded to diet and were classified as having food-responsive enteropathy. In 13 dogs a final diagnosis was not established. MAP-specific DNA was detected and confirmed by sequencing in 8 dogs (19%). These dogs were diagnosed with food-responsive enteropathy (n=3), IBD (n=2), and open diagnosis (n=3). MAP-specific DNA was not detected in dogs with no gastrointestinal disease.

CONCLUSIONS AND CLINICAL IMPORTANCE:

MAP-specific DNA was detected in approximately one fifth of dogs with chronic gastrointestinal disease and might play a role as a pathogenic agent. Apart from animal welfare, the zoonotic aspect warrants further studies addressing the viability of MAP organism in canine intestinal biopsies by culture.

PMID:
18638019
DOI:
10.1111/j.1939-1676.2008.0147.x
[Indexed for MEDLINE]
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