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Circ Res. 2008 Aug 15;103(4):340-2. doi: 10.1161/CIRCRESAHA.108.182469. Epub 2008 Jul 17.

Myofibrillar architecture in engineered cardiac myocytes.

Author information

1
Disease Biophysics Group, School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA. kkparker@ seas.harvard.edu

Abstract

Morphogenesis is often considered a function of transcriptional synchrony and the spatial limits of diffusing mitogens; however, physical constrainment by the cell microenvironment represents an additional mechanism for regulating self-assembly of subcellular structures. We asked whether myocyte shape is a distinct signal that potentiates the organization of myofibrillar arrays in cardiac muscle myocytes. We engineered the shape of neonatal rat ventricular myocytes by culturing them on microfabricated fibronectin islands, where they spread and assumed the shape of the island. Myofibrillogenesis followed, both spatially and temporally, the assembly of unique actin networks whose architecture was predictable given the shape of the island. Subsequently, the z lines of the sarcomeres aligned and registered in distinct patterns in different regions of the myocytes in such a way that orthogonal axes of contraction could be distinctly engineered. These data suggest that physical constrainment of muscle cells by extracellular matrix may be an important regulator of myofibrillar organization.

PMID:
18635822
PMCID:
PMC3910252
DOI:
10.1161/CIRCRESAHA.108.182469
[Indexed for MEDLINE]
Free PMC Article

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