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Methods Mol Biol. 2008;422:31-49. doi: 10.1007/978-1-59745-581-7_3.

FISH for mapping single copy genes.

Author information

1
Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.

Abstract

During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.

PMID:
18629659
DOI:
10.1007/978-1-59745-581-7_3
[Indexed for MEDLINE]

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