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Biotechnol Lett. 2008 Dec;30(12):2125-30. doi: 10.1007/s10529-008-9799-x. Epub 2008 Jul 16.

Simultaneous degradation of phytic acid and starch by an industrial strain of Saccharomyces cerevisiae producing phytase and alpha-amylase.

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1
Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, South Korea.

Abstract

Phytase liberates inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) which is the major phosphate reserve in plant-derived foods and feeds. An industrial strain of Saccharomyces cerevisiae expressing the Debaryomyces castellii phytase gene (phytDc) and D. occidentalis alpha-amylase gene (AMY) was developed. The phytDc and AMY genes were constitutively expressed under the ADC1 promoter in S. cerevisiae by using the delta-integration system, which contains DNA derived exclusively from yeast. The recombinant industrial strain secreted both phytase and alpha-amylase for the efficient degradation of phytic acid and starch as main components of plant seeds. This new strain hydrolyzed 90% of 0.5% (w/v) sodium phytate within 5 days of growth and utilized 100% of 2% (w/v) starch within 48 h simultaneously.

PMID:
18629438
DOI:
10.1007/s10529-008-9799-x
[Indexed for MEDLINE]
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