Imaging technologies for in vivo functional and molecular imaging in small animals have undergone a very fast development in the last years with very intense competition to further develop resolution and molecular sensitivity. Among the imaging technologies available, ultrasound-based molecular imaging methods are of particular interest, since the use of ultrasound contrast agents allows specific and sensitive depiction of molecular targets. Together with new developments in quantification methods of targeted microbubbles, sonography represents a dynamic and seminal tool for molecular imaging.