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Nat Methods. 2008 Aug;5(8):683-5. doi: 10.1038/nmeth.1235. Epub 2008 Jul 11.

Improving membrane voltage measurements using FRET with new fluorescent proteins.

Author information

1
Laboratory for Cell Function Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan.

Abstract

We used two new coral fluorescent proteins as fluorescence resonance energy transfer (FRET) donor and acceptor to develop a voltage sensor, named Mermaid, that displays approximately 40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells. Notably, Mermaid has fast on-off kinetics at warm (approximately 33 degrees C) temperatures and can report voltage spikes comparable to action potentials.

PMID:
18622396
DOI:
10.1038/nmeth.1235
[Indexed for MEDLINE]

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