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Nucleic Acids Res. 1991 Jul 25;19(14):3887-91.

K-tuple frequency in the human genome and polymerase chain reaction.

Author information

1
Laboratoire des Chlamydiales et des Rickettsiales, Institut Pasteur, Paris, France.

Abstract

The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers to amplify DNA target sequences, either of human genes or of human infectious agents. The software also gave nested primer sequences which were used to synthesize non radioactive probes by PCR. We applied these two methods, primer selection and non radioactive probes, to easily and quickly set up very efficient PCR sets to work in the human genome context.

PMID:
1861980
PMCID:
PMC328479
DOI:
10.1093/nar/19.14.3887
[Indexed for MEDLINE]
Free PMC Article

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