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Curr Opin Biotechnol. 2008 Aug;19(4):316-23. doi: 10.1016/j.copbio.2008.06.005. Epub 2008 Jul 23.

Two-hybrid technologies in proteomics research.

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Department of Biochemistry and Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research (DCCBR), University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.


Given that protein-protein interactions (PPIs) regulate nearly every living process; the exploration of global and pathway-specific protein interaction networks is expected to have major implications in the understanding of diseases and for drug discovery. Consequently, the development and application of methodologies that address physical associations among proteins is of major importance in today's proteomics research. The most widely and successfully used methodology to assess PPIs is the yeast two-hybrid system (YTH). Here we present an overview on the current applications of YTH and variant technologies in yeast and mammalian systems. Two-hybrid-based methods will not only continue to have a dominant role in the assessment of protein interactomes but will also become important in the development of novel compounds that target protein interaction interfaces for therapeutic intervention.

[Indexed for MEDLINE]

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