Format

Send to

Choose Destination
See comment in PubMed Commons below
Auris Nasus Larynx. 2009 Apr;36(2):152-6. doi: 10.1016/j.anl.2008.05.005. Epub 2008 Jul 7.

Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea.

Author information

1
Department of Otolaryngology, Kyushu University, Higashi-ku, Fukuoka, Japan. kimitaka@qent.med.kyushu-u.ac.jp

Abstract

OBJECTIVES:

The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs).

METHODS:

IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10mmol/L sodium salicylate solutions or 0.01mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells.

RESULTS:

Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20min and in 40min, whereas OHCs were shortened by the 10mmol/L sodium salicylate to 83% in 20min and 75% in 40min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20min and 40min both in IHCs and OHCs.

CONCLUSIONS:

Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.

PMID:
18606508
DOI:
10.1016/j.anl.2008.05.005
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center