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J Am Chem Soc. 2008 Jul 30;130(30):9638-9. doi: 10.1021/ja802355u. Epub 2008 Jul 8.

A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells.

Author information

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

Erratum in

  • J Am Chem Soc. 2008 Aug 27;130(34):11561.

Abstract

We present the design, synthesis, and biological applications of mitochondria peroxy yellow 1 (MitoPY1), a new type of bifunctional fluorescent probe for imaging hydrogen peroxide levels within the mitochondria of living cells. MitoPY1 combines a chemoselective boronate-based switch and a mitochondrial-targeting phosphonium moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal microscopy and flow cytometry experiments in a variety of mammalian cell types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide concentrations generated by situations of oxidative stress.

PMID:
18605728
PMCID:
PMC2810491
DOI:
10.1021/ja802355u
[PubMed - indexed for MEDLINE]
Free PMC Article
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