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Neuroreport. 2008 Jul 16;19(11):1131-5. doi: 10.1097/WNR.0b013e328305639d.

Nandrolone decreases mu opioid receptor expression in SH-SY5Y human neuroblastoma cells.

Author information

1
Department of Pharmacology, University of Bologna, Bologna, Italy.

Abstract

Nandrolone and other anabolic androgenic steroids alter the expression and function of neurotransmitter systems and contribute to drug dependence. Nandrolone treatment (10-10 M) caused a time-dependent and concentration-dependent downregulation of mu opioid receptor (MOPr) transcripts in SH-SY5Y human neuroblastoma cells. This effect was prevented by the androgen receptor antagonist hydroxyflutamide. Receptor binding assays confirmed a decrease in MOPr of approximately 40% in nandrolone-treated cells. Treatment with actinomycin D (10 (-5)M), a transcription inhibitor, revealed that nandrolone might regulate MOPr mRNA stability. In SH-SY5Y cells transfected with a human MOPr luciferase promoter/reporter construct, nandrolone did not alter the rate of gene transcription. These results suggest that nandrolone may regulate MOPr expression through posttranscriptional mechanisms requiring the androgen receptor.

PMID:
18596614
DOI:
10.1097/WNR.0b013e328305639d
[Indexed for MEDLINE]

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