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Arch Biochem Biophys. 2008 Sep 1;477(1):33-42. doi: 10.1016/j.abb.2008.06.006. Epub 2008 Jun 19.

Specific regulation of CYP27B1 and VDR in proximal versus distal renal cells.

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1
Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298-0614, USA.

Abstract

In this study, we utilized murine renal proximal (MPCT-G) and distal (DKC-8) tubular epithelial cell lines to compare the gene expressions and promoter activities of 1,25(OH)(2)D(3) receptor (VDR) and 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1) in response to 50 nM of parathyroid hormone (PTH) and changes in extracellular calcium (Ca(2+)) concentration. In MPCT-G cells, VDR gene expression was suppressed by PTH, whereas CYP27B1 gene expression was elevated in response to PTH. In DKC-8 cells, treatment of PTH significantly increased the relative gene expression of VDR by 6.5-fold while CYP27B1 gene expression was unchanged. High Ca(2+) exposure stimulated VDR gene expression and repressed CYP27B1 gene expression in both dose and time-dependent fashion in MPCT-G but not DKC-8 cells. The analysis of promoter activities and VDR protein levels corresponded with the gene expression data. We conclude that PTH-mediated decrease in VDR and increase in renal CYP27B1 is proximal cell-specific.

PMID:
18593564
DOI:
10.1016/j.abb.2008.06.006
[Indexed for MEDLINE]
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