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Methods Mol Biol. 2008;484:263-76. doi: 10.1007/978-1-59745-398-1_17.

Identification and characterization of N-glycosylated proteins using proteomics.

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1
Protein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.

Abstract

Glycoproteins constitute a large fraction of the proteome. The fundamental role of protein glycosylation in cellular development, growth, and differentiation, tissue development, and in host-pathogen interactions is by now widely accepted. Proteome-wide characterization of glycoproteins is a complex task and is currently achieved by mass spectrometry-based methods that enable identification of glycoproteins and localization, classification, and analysis of individual glycan structures on proteins. In this chapter we briefly introduce a range of analytical technologies for recovery and analysis of glycoproteins and glycopeptides. Combinations of affinity-enrichment techniques, chemical and biochemical protocols, and advanced mass spectrometry facilitate detailed glycoprotein analysis in proteomics, from fundamental biological studies to biomarker discovery in biomedicine.

PMID:
18592185
DOI:
10.1007/978-1-59745-398-1_17
[Indexed for MEDLINE]
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