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Antimicrob Agents Chemother. 2008 Sep;52(9):3424-6. doi: 10.1128/AAC.00462-08. Epub 2008 Jun 30.

Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay.

Author information

1
Department of Research and Development, Antimicrobial Resistance Division, Medical Diagnostic Laboratories, LLC, 2439 Kuser Road, Hamilton, NJ 08690, USA. sgygax@mdlab.com

Abstract

A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a >or=3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

PMID:
18591262
PMCID:
PMC2533493
DOI:
10.1128/AAC.00462-08
[Indexed for MEDLINE]
Free PMC Article

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