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Curr Opin Biotechnol. 2008 Aug;19(4):331-7. doi: 10.1016/j.copbio.2008.06.001. Epub 2008 Jul 11.

High confidence determination of specific protein-protein interactions using quantitative mass spectrometry.

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Department of proteomics and signal transduction, Max-Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.


In recent years, interactions between proteins have successfully been determined by mass spectrometry. A limitation of this technology has been the need for extensive purification, which restricts throughput and implies a tradeoff between specificity and the ability to detect weak or transient interactions. Quantitative proteomics sidesteps this problem by directly comparing specific and control pull-downs. Specific interaction partners are revealed by their quantitative ratios rather than by gel-based visualization and can be retrieved from a vast excess of background proteins. This principle is revolutionizing the protein interaction field as demonstrated by recent applications in fields as diverse as tyrosine signaling pathways, cell adhesion, and chromatin biology.

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