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Biotechnol Bioeng. 1988 Mar;31(4):321-7.

A microfibril-generating factor from the cellulase of Trichoderma reesei.

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Northern Regional Research Center, Agricultural Research Service, U.S. Department of AgricultureThe mention of firm names or trade products does not imly that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned, Peoria, Illinois 61604.


A combination of ionic strength reduction and diafiltration of Trichoderma reesei cellulate complex through a hollow fiber apparatus of 5000 molecular weight (MW) cutoff and subsequent passage of filtrate over a Spherogel-TSK 3000-SW column provided extracts that had the ability to generate microfibrils in filter paper and to disrupt filter paper and corn leaf tissue. Milligram quantities of material obtained from these extracts released small amounts of soluble carbohydrate from filter paper, required ferric iron for increased activity, and contained amino acids. Short fiber formation and disruption of filter paper during interaction with these extracts was enhanced by prior acid treatment and eliminated by prior base treatment. The amount of soluble carbohydrate hydrolyzed in 24 h from filter paper by whole cellulase complex was not changed by first disrupting the substrate with the extracts.


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