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Matrix Biol. 2008 Jul;27(6):505-12. doi: 10.1016/j.matbio.2008.05.002. Epub 2008 May 27.

Col2-Cre recombinase is co-expressed with endogenous type II collagen in embryonic renal epithelium and drives development of polycystic kidney disease following inactivation of ciliary genes.

Author information

1
Department of Developmental Biology, Harvard School of Dental Medicine, Boston, Massachusetts 02115, USA. elona_kolpakova@hms.harvard.edu

Abstract

Here we report on the severe defects in renal epithelium induced by the transgenic Col2-Cre line used previously for skeletal tissue-specific gene targeting. We demonstrate that conditional ablation of the Kif3a or Pkd1 genes encoding primary cilium/intraflagellar transport-associated proteins using type II collagen-specific Cre transgenic strain results in a severe form of polycystic kidney disease in mice. We detect Col2-Cre recombinase expression in kidney epithelium, which reflects expression of the endogenous Col1alpha(II) gene in the embryonic renal tubules. We determine the exon 2-containing splice variant of the Col1alpha(II) gene as a major transcript expressed in kidney. Furthermore, the confocal immunocytochemical analysis demonstrates deposition of the type II collagen within the mesenchymal-epithelial renal tissue interfaces and its co-localization with the basement membrane marker collagen IV during embryonic kidney morphogenesis.

PMID:
18579360
PMCID:
PMC2564992
DOI:
10.1016/j.matbio.2008.05.002
[Indexed for MEDLINE]
Free PMC Article

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