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Mol Biotechnol. 2008 Oct;40(2):195-201. doi: 10.1007/s12033-008-9079-y. Epub 2008 Jun 25.

Cloning of the thermostable cellulase gene from newly isolated Bacillus subtilis and its expression in Escherichia coli.

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College of Animal Sciences, Northwest A&F University, Yangling, 712100, People's Republic of China.


A bacterial strain with high cellulase activity (0.26 U/ml culture medium) was isolated from hot spring, and classified and named as B. subtilis DR by morphological and 16SrDNA gene sequence analysis. A thermostable endocellulase, CelDR, was purified from the isolated strain. The optimum temperature of the enzyme reaction was 50 degrees C, and CelDR retained 70% of its maximum activity at 75 degrees C after incubation for 30 min. The putative gene celDR, consisting an open reading frame (ORF) of 1,524 nucleotides and encoding a protein of 508 amino acids with a molecular weight of 55 kDa, was purified from B. subtilis DR and cloned into pET-28a for expression. The cellulase production in E. coli BL21 (DE3) was enhanced to approximately three times that of the wild-type strain.

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