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PLoS One. 2008 Jun 25;3(6):e2514. doi: 10.1371/journal.pone.0002514.

Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.

Author information

1
Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako-City, Saitama, Japan.

Abstract

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

PMID:
18575613
PMCID:
PMC2429971
DOI:
10.1371/journal.pone.0002514
[Indexed for MEDLINE]
Free PMC Article

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