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J Invertebr Pathol. 2008 Sep;99(1):8-13. doi: 10.1016/j.jip.2008.04.010. Epub 2008 May 6.

Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB).

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1
Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Kyonggi-do, Republic of Korea.

Abstract

A novel micro-PCR-based detection method, termed ultra-rapid real-time PCR, was applied to the development of a rapid detection for Paenibacillus larvae (P. larvae) which is the causative agent of American Foulbrood (AFB). This method was designed to detect the 16S rRNA gene of P. larvae with a micro-scale chip-based real-time PCR system, GenSpector TMC-1000, which has uncommonly fast heating and cooling rates (10 degrees C per second) and small reaction volume (6microl). In the application of ultra-rapid real-time PCR detection to an AFB-infected larva, the minimum detection time was 7 min and 54s total reaction time (30 cycles), including the melting temperature analysis. To the best of our knowledge, this novel detection method is one of the most rapid real-time PCR-based detection tools.

PMID:
18571197
DOI:
10.1016/j.jip.2008.04.010
[Indexed for MEDLINE]
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