Format

Send to

Choose Destination
Curr Eye Res. 2008 May;33(5):507-15. doi: 10.1080/02713680802110208.

Dexamethasone increases pigment epithelium-derived factor in perfused human eyes.

Author information

1
Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, NIH, Bethesda, Maryland, USA.

Abstract

PURPOSE:

To investigate the effects of dexamethasone (DEX) on pigment epithelium-derived factor (PEDF) cDNA and secreted protein in human trabecular meshwork (TM).

METHODS:

Anterior segment organ cultures were perfused with 0.1 microM DEX (OD) and vehicle (OS). Primary human TM cells (HTM) were treated with DEX under similar conditions. PEDF mRNA and secreted PEDF protein were quantitated by RT-PCR and Western blot.

RESULTS:

PEDF mRNA and secreted PEDF protein levels were significantly higher in DEX over vehicle-treated cultures. In contrast, DEX decreased the activity of a 92-kDa gelatinolytic zymogen in organ culture effluents.

CONCLUSION:

DEX action in the human TM might include a PEDF-mediating pathway.

PMID:
18568889
PMCID:
PMC2720275
DOI:
10.1080/02713680802110208
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center