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Org Biomol Chem. 2008 Jul 7;6(13):2308-15. doi: 10.1039/b803258k. Epub 2008 May 12.

N-Myristoyl transferase-mediated protein labelling in vivo.

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1
Department of Chemistry, South Kensington Campus, Imperial College, London, UK SW7 2AZ.

Abstract

N-Myristoyl transferase-mediated labelling using a substrate modified with an azide or alkyne tag is described as an efficient and site-selective method for the introduction of a bioorthogonal tag at the N-terminus of a recombinant protein. The procedure may be performed in vitro, or in a single over-expression/tagging step in vivo in bacteria; tagged proteins may then be captured using Staudinger-Bertozzi or 'click' chemistry protocols to introduce a secondary label for downstream analysis. The straightforward synthesis of the chemical and molecular biological tools described should enable their use in a wide range of N-terminal labelling applications.

PMID:
18563263
DOI:
10.1039/b803258k
[Indexed for MEDLINE]
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