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PLoS One. 2008 Jun 18;3(6):e2382. doi: 10.1371/journal.pone.0002382.

A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus.

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1
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.

Abstract

Alpha-herpesviruses, including herpes simplex virus and pseudorabies virus (PRV), infect the peripheral nervous system (PNS) of their hosts. Here, we describe an in vitro method for studying neuron-to-cell spread of infection as well as viral transport in axons. The method centers on a novel microfluidic chamber system that directs growth of axons into a fluidically isolated environment. The system uses substantially smaller amounts of virus inoculum and media than previous chamber systems and yet offers the flexibility of applying multiple virology and cell biology assays including live-cell optical imaging. Using PRV infection of cultured PNS neurons, we demonstrate that the microfluidic chamber recapitulates all known facets of neuron-to-cell spread demonstrated in animals and other compartmented cell systems.

PMID:
18560518
PMCID:
PMC2426917
DOI:
10.1371/journal.pone.0002382
[Indexed for MEDLINE]
Free PMC Article
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