Format

Send to

Choose Destination
See comment in PubMed Commons below
Nano Lett. 2008 Jul;8(7):2063-9. doi: 10.1021/nl801483w. Epub 2008 Jun 18.

Induction of cell polarization and migration by a gradient of nanoscale variations in adhesive ligand spacing.

Author information

  • 1Department of New Materials and Biosystems, Max Planck Institute for Metals Research, and Department of Biophysical Chemistry, University of Heidelberg, Heisenbergstr. 3, D-70569 Stuttgart, Germany.

Abstract

Cell interactions with adhesive surfaces play a vital role in the regulation of cell proliferation, viability, and differentiation, and affect multiple biological processes. Since cell adhesion depends mainly on the nature and density of the adhesive ligand molecules, spatial molecular patterning, which enables the modulation of adhesion receptor clustering, might affect both the structural and the signaling activities of the adhesive interaction. We herein show that cells plated on surfaces that present a molecularly defined spacing gradient of an integrin RGD ligand can sense small but consistent differences in adhesive ligand spacing of about 1 nm across the cell diameter, which is approximately 61 mum when the spacing includes 70 nm. Consequently, these positional cues induce cell polarization and initiate cell migration and signaling. We propose that differential positional clustering of the integrin transmembrane receptors is used by cells for exploring and interpreting their environment, at high spatial sensitivity.

PMID:
18558788
PMCID:
PMC3811077
DOI:
10.1021/nl801483w
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Support Center