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Res Microbiol. 2008 Jul-Aug;159(6):476-80. doi: 10.1016/j.resmic.2008.04.001. Epub 2008 Apr 13.

Pathogen-derived nitric oxide influences formation of the appressorium infection structure in the phytopathogenic fungus Blumeria graminis.

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Institute of Sustainable Agriculture-CSIC, Alameda del Obispo, Menéndez Pidal s/n, 14080 Córdoba, Spain.


Nitric oxide (NO) is an important signal in plant resistance to pathogens. Here we report that NO is also generated by Blumeria graminis f.sp. hordei as a pathogenesis determinant on barley. Infection by B. graminis f.sp. hordei is dependent on appressorium formation in order to penetrate the host. Using fluorescent dye diaminofluorescein-2 diacetate (DAF-2DA) and confocal laser scanning microscopy, transient NO generation was detected within the B. graminis f.sp. hordei appressorium during its maturation. To confirm that NO was indeed being measured, DAF-2DA fluorescence was suppressed using a NO scavenger and a mammalian NO synthase inhibitor. Both chemicals affected the number of appressorial lobes produced by the fungus. These data indicate that NO plays a key role in formation of B. graminis f.sp. hordei appressoria.

[Indexed for MEDLINE]

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