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Zebrafish. 2008 Summer;5(2):121-3. doi: 10.1089/zeb.2008.9988.

Zinc finger-based knockout punches for zebrafish genes.

Author information

1
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA. Ekker.Stephen@mayo.edu

Abstract

The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.

PMID:
18554175
PMCID:
PMC2849655
DOI:
10.1089/zeb.2008.9988
[Indexed for MEDLINE]
Free PMC Article
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