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Curr Protoc Nucleic Acid Chem. 2008 Jun;Chapter 6:Unit 6.10. doi: 10.1002/0471142700.nc0610s33.

Bisulfite modification for analysis of DNA methylation.

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1
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama, Japan.

Abstract

Bisulfite is known to deaminate cytosine in nucleic acids, while 5-methylcytosine resists this bisulfite action. For this reason, bisulfite treatment has been used for detecting 5-methylcytosine in DNA, a minor component of eukaryotic DNA, presently recognized as playing an important role in the control of gene function. This procedure, called bisulfite genomic sequencing, is a principal method for the analysis of DNA methylation in various biological phenomena, including human diseases such as cancer. This unit describes an efficient procedure utilizing a newly developed high-concentration bisulfite solution. Protocols for this methodology are supplemented with discussions focused on chemical aspects of the bisulfite treatment.

PMID:
18551428
DOI:
10.1002/0471142700.nc0610s33
[Indexed for MEDLINE]
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