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Hum Reprod. 2008 Sep;23(9):2151-9. doi: 10.1093/humrep/den191. Epub 2008 Jun 10.

Polymorphisms in the human cysteine-rich secretory protein 2 (CRISP2) gene in Australian men.

Author information

1
Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, VIC, Australia.

Abstract

BACKGROUND:

Cysteine-rich secretory protein 2 (CRISP2) is localized to the human sperm acrosome and tail. It can regulate ryanodine receptors Ca(2+) gating and binds to mitogen-activated protein kinase kinase kinase 11 in the acrosome and gametogenetin 1 (GGN1) in the tail.

METHODS AND RESULTS:

In order to test the hypothesis that CRISP2 variations contribute to male infertility, we screened coding and flanking intronic regions in 92 infertile men with asthenozoo- and/or teratozoospermia and 176 control men using denaturing HPLC and sequencing. There were 21 polymorphisms identified, including 13 unreported variations. Three SNPs resulted in amino acid substitutions: L59V, M176I and C196R. All were only present in a heterozygous state and found in fertile men. However, the C196R polymorphism was of particular interest as it resulted in the loss of a strictly conserved cysteine involved in intramolecular disulphide bonding. Screening of an additional 637 infertile men identified 23 heterozygous C196R men to give an overall frequency of 3.6%, compared with 3.4% in control men. The functional significance of the C196R polymorphism was defined using a yeast two-hybrid assay. The C196R substitution resulted in the loss of CRISP2-GGN1 binding.

CONCLUSIONS:

Although none of the many polymorphisms identified herein showed a significant association with male infertility, functional studies suggested that the C196R polymorphism may compromise CRISP2 function.

PMID:
18550510
DOI:
10.1093/humrep/den191
[Indexed for MEDLINE]

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