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Opt Express. 2008 Jun 9;16(12):8670-7.

Simple and robust image-based autofocusing for digital microscopy.

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1
GE Global Research, One Research Circle, Niskayuna, N.Y. 12309, USA. siavash.yazdanfar@research.ge.com

Abstract

A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.

PMID:
18545580
[Indexed for MEDLINE]
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