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Anal Biochem. 2008 Sep 1;380(1):146-8. doi: 10.1016/j.ab.2008.05.020. Epub 2008 May 22.

A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells.

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1
Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria.

Abstract

Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.

PMID:
18541133
DOI:
10.1016/j.ab.2008.05.020
[Indexed for MEDLINE]
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