The yeast checkpoint kinase Dun1 downregulates DIN7 in the absence of DNA damage

Ayako Yoshitani; Feng Ling; Minoru Yoshida

doi:10.1271/bbb.80114

The yeast checkpoint kinase Dun1 downregulates DIN7 in the absence of DNA damage

Biosci Biotechnol Biochem. 2008 Jun;72(6):1630-4. doi: 10.1271/bbb.80114. Epub 2008 Jun 7.

Authors

Ayako Yoshitani¹, Feng Ling, Minoru Yoshida

Affiliation

¹ Chemical Genetics Laboratory, RIKEN Discovery Research Institute, Wako-shi, Saitama 351-0198, Japan.

PMID: 18540090
DOI: 10.1271/bbb.80114

Abstract

Yeast DIN7 is a DNA damage-inducible gene. Its expression is increased in the absence of Dun1, a DNA damage checkpoint kinase. We identified a DIN7 promoter region responsible for Dun1-mediated downregulation and found that DIN7 expression was not further increased in response to hydroxyurea in Deltadun1 cells. Thus DIN7 repression by Dun1 can be released upon DNA damage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
DNA Damage / genetics
Down-Regulation*
Exodeoxyribonucleases / genetics
Exodeoxyribonucleases / metabolism*
Gene Expression Regulation, Fungal*
Promoter Regions, Genetic / genetics
Protein Kinases / genetics
Protein Kinases / metabolism*
Protein Serine-Threonine Kinases
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*

Substances

Cell Cycle Proteins
Saccharomyces cerevisiae Proteins
Protein Kinases
DUN1 protein, S cerevisiae
Protein Serine-Threonine Kinases
DIN7 protein, S cerevisiae
Exodeoxyribonucleases