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Dev Cell. 2008 Jun;14(6):843-53. doi: 10.1016/j.devcel.2008.03.012.

MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors.

Author information

1
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Abstract

Lineage specification is a critical issue in developmental and regenerative biology. We hypothesized that microRNAs (miRNAs) are important participants in those processes and used the poorly understood regulation of megakaryocyte-erythrocyte progenitors (MEPs) in hematopoiesis as a model system. We report here that miR-150 modulates lineage fate in MEPs. Using a novel methodology capable of profiling miRNA expression in small numbers of primary cells, we identify miR-150 as preferentially expressed in the megakaryocytic lineage. Through gain- and loss-of-function experiments, we demonstrate that miR-150 drives MEP differentiation toward megakaryocytes at the expense of erythroid cells in vitro and in vivo. Moreover, we identify the transcription factor MYB as a critical target of miR-150 in this regulation. These experiments show that miR-150 regulates MEP fate, and thus establish a role for miRNAs in lineage specification of mammalian multipotent cells.

PMID:
18539114
PMCID:
PMC2688789
DOI:
10.1016/j.devcel.2008.03.012
[Indexed for MEDLINE]
Free PMC Article

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