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Nat Methods. 2008 Jul;5(7):605-7. doi: 10.1038/nmeth.1220. Epub 2008 Jun 8.

Lifeact: a versatile marker to visualize F-actin.

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1
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.

Abstract

Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.

PMID:
18536722
PMCID:
PMC2814344
DOI:
10.1038/nmeth.1220
[Indexed for MEDLINE]
Free PMC Article
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