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Virology. 1991 Aug;183(2):658-76.

Genome organization of membrane-containing bacteriophage PRD1.

Author information

1
Department of Genetics, University of Helsinki, Finland.

Abstract

We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome.

PMID:
1853567
DOI:
10.1016/0042-6822(91)90995-n
[Indexed for MEDLINE]

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