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Expert Rev Proteomics. 2008 Jun;5(3):435-44. doi: 10.1586/14789450.5.3.435.

LC-MS for protein characterization: current capabilities and future trends.

Author information

1
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. guodong.chen@spcorp.com

Abstract

With advances in ionization methods and instrumentation, liquid chromatography (LC)/mass spectrometry (MS) has become a powerful technology for protein characterization. This review article will describe the general approaches on LC-MS analysis in protein characterization, including bottom-up and top-down strategies. Discussions will be given on characterization of recombinant proteins, and post-translational and protein modifications such as disulfide bonds, glycosylation and phosphorylation using LC-MS. New research directions in this area will also be presented to illustrate future prospects of LC-MS in protein characterization, including application to proteomics.

PMID:
18532911
DOI:
10.1586/14789450.5.3.435
[Indexed for MEDLINE]

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