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Photochem Photobiol Sci. 2008 Jun;7(6):668-70. doi: 10.1039/b800391b. Epub 2008 Apr 1.

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.

Author information

1
Research Institute for Electronic Science, Hokkaido University, Sapporo, 060-0812, Japan.

Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

PMID:
18528549
DOI:
10.1039/b800391b
[Indexed for MEDLINE]

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