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Inflamm Res. 2008 Jun;57(6):272-8. doi: 10.1007/s00011-007-7131-1.

Role of lymphocytes in the course of murine zymosan-induced peritonitis.

Author information

1
Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, PL-30-060 Krakow, Poland. ela.kolaczkowska@uj.edu.pl

Abstract

OBJECTIVE AND DESIGN:

To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.

MATERIAL OR SUBJECTS:

Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).

TREATMENT:

Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.

METHODS:

At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk's staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE(2), KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-gamma, TNF-alpha, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student's t-test (p </=0.05).

RESULTS:

During zymosan peritonitis, there was a decreased production of IFN-alpha (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.

CONCLUSIONS:

Lymphocytes do not have a significant impact on zymosan peritonitis in mice.

PMID:
18516709
DOI:
10.1007/s00011-007-7131-1
[Indexed for MEDLINE]
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