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Bioorg Med Chem Lett. 2008 Jul 1;18(13):3749-51. doi: 10.1016/j.bmcl.2008.05.046. Epub 2008 May 16.

Design of a fluorescence polarization assay platform for the study of human Hsp70.

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1
Program in Molecular Pharmacology and Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Abstract

The 70kDa heat shock proteins (Hsp70) are molecular chaperones that assist in folding of newly synthesized polypeptides, refolding or denaturation of misfolded proteins, and translocation of proteins across biological membranes. In addition, Hsp70 play regulatory roles in signal transduction, cell cycle, and apoptosis. Here, we present a novel assay platform based on fluorescence polarization that is suitable for investigating the yet elusive molecular mechanics of human Hsp70 allosteric regulation.

PMID:
18515098
PMCID:
PMC2538440
DOI:
10.1016/j.bmcl.2008.05.046
[Indexed for MEDLINE]
Free PMC Article
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