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Biochem Biophys Res Commun. 2008 Aug 8;372(4):752-5. doi: 10.1016/j.bbrc.2008.05.100. Epub 2008 Jun 2.

Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging.

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Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1PD, UK.


There has been confusion about the subunit stoichiometry of the degenerin family of ion channels. Recently, a crystal structure of acid-sensing ion channel (ASIC) 1a revealed that it assembles as a trimer. Here, we used atomic force microscopy (AFM) to image unprocessed ASIC1a bound to mica. We detected a mixture of subunit monomers, dimers and trimers. In some cases, triple-subunit clusters were clearly visible, confirming the trimeric structure of the channel, and indicating that the trimer sometimes disaggregated after adhesion to the mica surface. This AFM-based technique will now enable us to determine the subunit arrangement within heteromeric ASICs.

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