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Nat Methods. 2008 Jun;5(6):491-505. doi: 10.1038/nmeth.1218.

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

Author information

1
Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, 50 South Drive, Bethesda, Maryland 20892, USA. neumankc@mail.nih.gov

Abstract

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.

PMID:
18511917
PMCID:
PMC3397402
DOI:
10.1038/nmeth.1218
[Indexed for MEDLINE]
Free PMC Article

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