Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2008 Jun;5(6):475-89. doi: 10.1038/nmeth.1215.

Do-it-yourself guide: how to use the modern single-molecule toolkit.

Author information

1
Department of Chemistry, Single Molecule Analysis Group, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA. nwalter@umich.edu

Abstract

Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethered particle microscopy, optical and magnetic tweezers, and atomic force microscopy. We also provide guidelines for choosing the right approach from the available single-molecule toolkit for applications as diverse as structural biology, enzymology, nanotechnology and systems biology.

PMID:
18511916
PMCID:
PMC2574008
DOI:
10.1038/nmeth.1215
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center