Format

Send to

Choose Destination
Magn Reson Med. 2008 Jun;59(6):1347-54. doi: 10.1002/mrm.21577.

AxCaliber: a method for measuring axon diameter distribution from diffusion MRI.

Author information

1
Department of Neurobiology, the George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. asafyan@zahav.net.il

Abstract

The diameter of a myelinated nerve axon is directly proportional to its conduction velocity, so the axon diameter distribution helps determine the channel capacity of nervous transmission along fascicles in the central (CNS) and peripheral nervous systems (PNS). Previously, this histological information could only be obtained using invasive tissue biopsies. Here we propose a new NMR-based approach that employs a model of water diffusion within "restricted" cylindrical axons to estimate their diameter distribution within a nerve bundle. This approach can be combined with MRI to furnish an estimate of the axon diameter distribution within each voxel. This method is validated by comparing the diameter distributions measured using the NMR and histological techniques on sciatic and optic nerve tissue specimens. The axon diameter distribution measured in each voxel of porcine spinal cord using MRI and using histological methods were similar. Applications are expected in longitudinal studies designed to follow nerve growth in normal and abnormal development, as well as in diagnosing disorders and diseases affecting specific populations of axons in the CNS and PNS.

PMID:
18506799
PMCID:
PMC4667732
DOI:
10.1002/mrm.21577
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center