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Biomed Chromatogr. 2008 Aug;22(8):912-7. doi: 10.1002/bmc.1030.

Analysis of iridoids from Harpagophytum and eleutherosides from Eleutherococcus senticosus in horse urine.

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1
Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370 Verrières le Buisson, France.

Abstract

LC/ESI-MS n methods have been previously set up to detect the administration of (i) Harpagophytum and (ii) preparations containing a plant capable of anti-stress properties: Eleutherococcus senticosus. Harpagoside has been found to be the main indicator of Harpagophytum administration in the horse. These methods have been applied to a large number of horse urine samples of various origins. Regarding the detection of Harpagophytum administration, harpagoside, harpagide and 8-para-coumaroyl harpagide were detected together in only one sample out of 317. Eleutheroside E was found to be the main indicator of Eleutherococcus senticosus administration. It was detected in post-administration samples collected from two horses having received a feed supplement containing Eleutherococcus senticosus for several days. Out of the 382 samples tested, eleutheroside E was found in an unexpected large number of urine samples (39%) of various origins and its presence cannot be only due to the sole use of herbal dietary supplements.

PMID:
18506730
DOI:
10.1002/bmc.1030
[Indexed for MEDLINE]

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