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Biochem Biophys Res Commun. 1991 Apr 15;176(1):479-85.

A region immediately adjacent to the origin of replication of bovine papilloma virus type 1 interacts in vitro with the nuclear matrix.

Author information

1
UnitĂ© de Recherches sur les Communications Hormonales, INSERM U-33, Hopital du Kremlin BicĂȘtre, France.

Abstract

We have investigated the interaction of the 69% transforming fragment of the Bovine Papilloma Virus type 1 (BPV1) with the nuclear matrix from 1361.5 cells (NIH-3T3 cells transformed by a BPV chimeric construct). In vitro studies performed with end-labelled DNA fragments and nuclear matrices prepared using a high-salt extraction procedure demonstrate the binding of a 672 bp fragment adjacent to the viral origin of replication and containing the plasmid maintenance sequence (PMS-1). This fragment can be cleaved into two pieces (393 and 279 bp), both interacting equally well with the nuclear matrix. This indicates that a least two regions of the 672 bp DNA fragment are involved in the interaction.

PMID:
1850268
DOI:
10.1016/0006-291x(91)90949-8
[Indexed for MEDLINE]

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