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Regul Pept. 2008 Nov 29;151(1-3):61-70. doi: 10.1016/j.regpep.2008.04.001. Epub 2008 Apr 8.

A new endogenous form of PYY isolated from canine ileum: Gly-extended PYY(1-36).

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CURE: Digestive Diseases Research Center, VA GLAHS, Los Angeles, CA 90073 and Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, United States.


We purified and identified the peptide YY (PYY) forms present and determined their levels from a portion of the canine ileum directly adjacent to the cecum by a new extraction method designed to prevent and evaluate degradation of endogenous peptides. We used three reverse phase chromatography steps with radioimmunoassay of fractions for PYY-like-immunoreactivity (PYY-LI). The purified fractions underwent intact protein/peptide mass spectrometry identification and sequencing (i.e. "top-down" MS analysis). This analysis confirmed the identity of a new form of PYY, PYY(1-36)-Gly, which co-elutes with PYY(1-36)-NH(2) through all three of separation steps used. The PYY(1-36)-Gly form represents approximately 20% of the total PYY found in this region of the canine intestine. In addition, we also found that the PYY(3-36)-NH(2) form represents 6% of the total PYY in the canine ileo-cecal junction. The physiological implication of the Gly-extended form of PYY(1-36) warrants further investigation.

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