Format

Send to

Choose Destination
Neurosci Bull. 2008 Jun;24(3):155-9.

Neuropeptide Y promotes TGF-beta1 production in RAW264.7 cells by activating PI3K pathway via Y1 receptor.

Author information

1
Department of Nautical Medicine, Laboratory of Stress Medicine, Second Military Medical University, Shanghai, China.

Abstract

OBJECTIVE:

To examine the effect of neuropeptide Y (NPY) on TGF-beta1 production in RAW264.7 macrophages.

METHODS:

Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-beta1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85.

RESULTS:

NPY treatment could promote TGF-beta1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-beta1 production induced by NPY could be abolished by wortmannin pretreatment.

CONCLUSION:

NPY may elicit TGF-beta1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.

PMID:
18500388
PMCID:
PMC5552544
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center