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J Vet Intern Med. 2008 Jul-Aug;22(4):886-92. doi: 10.1111/j.1939-1676.2008.0112.x.

Anti-erythrocyte antibodies and disease associations in anemic and nonanemic dogs.

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1
Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Abstract

BACKGROUND:

Flow cytometry has been used to detect anti-red blood cell (RBC) antibodies in dogs with immune-mediated hemolytic anemia (IMHA), but the prevalence of anti-RBC antibodies in anemic and nonanemic dogs with a variety of different diseases has not been assessed previously.

HYPOTHESIS:

We hypothesized that anti-RBC antibodies would be more common in anemic dogs and in dogs with immune-mediated disorders and cancer.

ANIMALS:

Blood samples from 292 dogs were analyzed prospectively by flow cytometry for anti-RBC antibodies.

METHODS:

Blood samples from 147 anemic and 145 nonanemic dogs were evaluated by flow cytometry to detect surface-bound immunoglobulin (Ig) G and IgM antibodies on RBC. Disease associations with RBC antibodies were determined, as was the correlation between disease status and the percentage of Ig(+) RBC. The specificity and sensitivity of flow cytometry and clinical variables for the diagnosis of IMHA were compared by Bayesian analysis.

RESULTS:

Anemic dogs were significantly more likely to be positive for anti-RBC antibodies (IgG, IgM, or both) than nonanemic dogs. Anemic dogs also had significantly higher percentages of Ig(+) RBC than nonanemic dogs, whereas dogs with IMHA had significantly higher percentages of Ig(+) RBC than dogs with all other diseases. Dogs with IMHA, infectious diseases, and immune-mediated thrombocytopenia were significantly more likely to have anti-RBC antibodies than dogs with other medical or surgical diseases.

CONCLUSIONS:

Anemic dogs with immune-mediated diseases and infectious diseases were at the highest risk for the development of anti-RBC antibodies, and flow cytometry for the detection of IgG on RBC was highly sensitive and specific for the diagnosis of IMHA.

PMID:
18498322
DOI:
10.1111/j.1939-1676.2008.0112.x
[Indexed for MEDLINE]
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