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Lab Chip. 2008 Jun;8(6):925-31. doi: 10.1039/b800721g. Epub 2008 Apr 11.

Microfluidic device for label-free measurement of platelet activation.

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1
Department of Physics, Macquarie University, Sydney, NSW 2109, Australia.

Abstract

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4 degrees C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

PMID:
18497913
DOI:
10.1039/b800721g
[Indexed for MEDLINE]
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