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Exp Hematol. 2008 Sep;36(9):1084-90. doi: 10.1016/j.exphem.2008.03.016. Epub 2008 May 20.

AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice.

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1
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.

Abstract

Fanconi anemia (FA) is a heterogeneous inherited disorder characterized by a progressive bone marrow (BM) failure and susceptibility to myeloid leukemia. Genetic correction using gene-transfer technology is one potential therapy. A major hurdle in applying this technology in FA patients is the inability of granulocyte colony-stimulating factor (G-CSF) to mobilize sufficient numbers of hematopoietic stem (HSC)/progenitor cells (HPC) from the BM to the peripheral blood. Whether the low number of CD34(+) cells is a result of BM hypoplasia or an inability of G-CSF to adequately mobilize FA HSC/HPC remains incompletely understood. Here we use competitive repopulation of lethally irradiated primary and secondary recipients to show that in two murine models of FA, AMD3100 synergizes with G-CSF resulting in a mobilization of HSC, whereas G-CSF alone fails to mobilize stem cells even in the absence of hypoplasia.

PMID:
18495331
PMCID:
PMC3877929
DOI:
10.1016/j.exphem.2008.03.016
[Indexed for MEDLINE]
Free PMC Article
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