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Chem Biol. 2008 May;15(5):513-9. doi: 10.1016/j.chembiol.2008.03.018.

Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.

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1
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.

Abstract

The addition of a hexahistidine tag to the N terminus of the hepatitis B capsid protein gives rise to a self-assembled particle with 80 sites of high local density of histidine side chains. Iron protoporphyrin IX has been found to bind tightly at each of these sites, making a polyvalent system of well-defined spacing between metalloporphyrin complexes. The spectroscopic and redox properties of the resulting particle are consistent with the presence of 80 site-isolated bis(histidine)-bound heme centers, comprising a polyvalent b-type cytochrome mimic.

PMID:
18482703
PMCID:
PMC2715361
DOI:
10.1016/j.chembiol.2008.03.018
[Indexed for MEDLINE]
Free PMC Article
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